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#1
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I just recently replaced the sand bed in my tank. Scooped out all the old sand and dumped in new sand (carefully of course). I've done this in the past too without any issues (and will likely continue to replace my sand every couple of years or so) aside from a short lived diatom bloom.
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#2
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I've just added sand to my already cycled tank 12 days ago and i haven't seen any negative effects on my critters/tank.just rinse the sand good before dumping it in...
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