Live Phytoplankton saltwater culture / HELP

[ScienceTeen]

Dear monocus, that's great that you confirmed how to make CO2! I have two stones to use, but thanks for offering.
I have another question (there may be a few more). We got the experiment underway, but, I realize that I'm not sure whether I have to do anything to the nanocloropus before adding CO2, i.e., siphoning off, adding of sea water, etc. Once there is evidence of phyto growth (how long will that be?), should I start a new bottle with salt water in it, and add only some of the phyto, or should I just start adding CO2 to the already growing culture? Please advise. Thanks.
ScienceTeen

[Xenoch88]

Where do you get starter cultures for these, and how do you grow them? do you have any good resources for this? I am looking into setting up a fuge for the sole purpose of growing pods, and different micro bacteria for the nutrition of my corals, and maybe, once it is setup and I get a chance, baby fish or what not. (also just cause I think it would be cool to do)

[monocus]

take the nanocloropus,add the same amount of water,and add a couple of drops of micro algae grow.start your experiment with your yeast culture co2 and don't add anymore micro algae grow.in another container do the same as above,but add a few drops of micro algae grow daily-you now have two cultures-1 being fed and the other relying on just co2.you can make a density stick for counting your cultures by using a small plastic ruler with a small 1/2" dot or stripe painted on the end-doesn't matter what colour.for every centimeter that you can see on the ruler before the dot dissapears will tell you how much your culture is growing.1 cm=27.6 2cm=19.2 3cm=13.3 4cm=8.8 5cm=5.2 6cm=2.9-these are millions of cells for nanochloropus.you can measure this daily and record your results,but wash off the ruler or better yet get two rulers and never mix your cultures.
i get my cultures from florida aqua farms and grow them in aquamedic reactors using led lights

[ScienceTeen]

Glad ScienceTeen asked. I'll pass this on, and she can get back to you with questions. She had a different idea of a control group, but this sounds like another good suggestion. She'll give you, consultant-on-tap extraordinaire, full credit!
ScienceMum

[BlueWorldAquatic]

Way too much information.

just use 2L coke bottles and G2 Fertilizer.

Water at 1.017, slow bubbles (one every 1/2 second, not to damage the cells), and lotsa light (Flouresent prefered)

Ken - BWA

[ponokareefer]

Originally Posted by monocus

i get my cultures from florida aqua farms and grow them in aquamedic reactors using led lights

I just got my "kit" from florida aqua farms after failing to grow phyto from a store boughten brand. The kit includes a 186 page manual that has a tone of good information. I would recommend going with them seeing how thorough this kit is.

[monocus]

store bought are 99% dead cultures.they have to be shaken at least twice a day so the cultures don't smother themselves.they are also a mix of various cultures so you wouldn't get a pure strain

[BlueWorldAquatic]

I beg to differ.

We actually propogate our own live cultures, that are also avaliable in bulk.

100% live.

[monocus]

sorry-i was referring to phyto that is packaged and sent to stores,not phyto that is cultured on the premises.