nannochloropsis phytoplankton culture (questions)

[phyto4life]

So far I started culturing nannochloropsis phytoplankton indoors using the petri dish method inwhich will be used to add directly to my saltwater tank that is at 1.024

I added a little less then 1/4 cup of rodi water that was boiled in the microwave for 1 min then I let it cool to room temp and added it to the petri dish then placed it under 2 normal output plant light's for 24 hours

where do I go from here?

I read that I should use the q-tip and add the entire dish to 1 liter of water under a gentle rolling boil and leave for 12-24 hours

my question is do I add this to fresh water or saltwater?

because ultimately I want the culture to be saltwater @ 1.020 ppm

and should I give the algae a break for a time period between adding the dish that was being lit for 24 hours to be lit in the 1L with flow for 16 hours?

I'll have more question's as I go