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Old 03-17-2011, 05:42 AM
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Liquid nitrogen stays liquid in insulated containers not under pressure - the rate of evporation is considerably lowered so that you need to replenish it only once every few weeks/months depending on how often the container is opened.

It's how we store cells and tissues for long term storage. Cryopreservation of mammalian cells is well established and routine - it's actually pretty easy to do, and you can store tissues and cells for decades.
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