propagating phytoplankton

[dreef]

Quote : Has anyone started a culture from a source that has been previously refrigerated?

I did, i worked just fine,don't know about dt's, i used some that a fellow reefer homebrews and sells.It was a month old, and refrigerated,just used a shotglass of it.Innoculated sterile 2L pop bottles filled with 0.19 saltwater,kept under 16 on,8 off light cycle, added a total of 3/4 tsp.of miracle grows.Let it go for 3 weeks and it turned out about 3 times darker green than what i purchased.