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					Originally Posted by reefwars  if your going with the  vinegar chart ( or vodka conversion)_....16 weeks is a bit over careful ...most do it in four weeks and similar to this
 0.1 ml  per gallon for 7 days
 0.2 ml per gallon for 7 days
 0.3 ml per gallon for 7 days
 0.4 ml per gallon for 7 days
 
 
 and going on up from there if needed , if nutrients are high to begin with then you can go as high as 1 ml per gallon , once your no3 and po4 drop to zero then you cut the dose back to a maintenance dose.
 
 most people have it by week 4 unless they were pretty high to begin with.
 
 no3 and po4 can become limiting in a closed system and bacteria need both in order to build mass so you may not hit zero if one or the other isnt available in numbers needed.
 
 cheers
 
 denny
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 Typically, you become limited by nitrate unless you are using GFO or lanthanum chloride. This is because of two things: bacteria use nitrogen in a high ratio as compared to phosphorus, and bacteria also convert some additional nitrate into nitrogen gas. Sometimes, though, some people have found they don't need to use a phosphate remover, or at least use a very small amount