Yeah density obviously decreases with temperature but at a rate of less than one tenth of a percent per 10 degrees C. So if you're close it really won't make a difference. Not to say you should use boiling or close to freezing temp water to calibrate but using say 21C water to calibrate for a tank running at 24C really won't make a detectable difference.
To give an idea, 4C RO water from my fridge reads about 0.5ppt on my refractometer, just barely over the zero line. And that's a pretty significant temperature difference.
Last edited by sphelps; 05-11-2012 at 05:33 PM.
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