Wait until the test kit no longer detects ammonia.
I believe Beverly is right - Prime will bind to ammonia converting it to a less harmful form. Many ammonia test kits, including API, test for total ammonia which includes this bound/converted ammonia. So your inhabitants may be safe even if the test kits reads > 0. Seachem has a "ammonia multi-test" kit that can measure both free ammonia and total ammonia.
Any ammonia, bound or not, indicates the cycle is still in progress.
Last edited by syncro; 01-06-2012 at 03:09 AM.
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