Quote:
Originally Posted by phyto4life
I posted early. tomorrow at 3pm I will have the petri dish with water under light for 24 hour's
from here you say to add it straight to a salinity of around 1.017 ppm with fertilizer and bubble the heck out of it in which my pump can do because it is set to bubble a 1 gallon
That is what I do.
my temp is probably around 70-75F
I don't think temp is a large factor, with higher temps you may get faster growth. I'm not sure, never worried about it, researched it or experimented.
my question is how much fertilizer and what amount of pre mixed saltwater/ liter/gallon etc to use right away?
the bottle from f.a.f liquid says 1/4 tsp for 1 G @ f/2 level and double that for full strength
should I go full strength fertilizer right away?
I would, it should help establish the best culture you possibly can.
and the petri dish says 1 liter for the entire dish to inoculate should I go more or the recommended amount at first right away?
I would dilute it, cultures are expensive. They will multiply rapidly. My qtip swab starts take about 3 weeks to become useful feed. When I start them they look like perfectly clear water.
ultimately I'll be doing 1G cultures x 8
I have a second dish so i'm open to what ever suggestions
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Start as many jugs as you want with the first dish. Save the second one for an emergency. If you have multiple cultures of the same species going it is highly unlikely you will lose them all to a crash at the same time. You can always restart a jug with phyto from another culture and still have an emergency backup dish. It really isn't difficult. I was overwhelmed by the info when I started too. It really is as easy as you want to make it.
I forgot to mention, I use miracle grow for fertilizer. The one with the smallest first and second numbers I can find. I started out using 5 ml in 2l of culture now I use 3 ml and haven't seen much difference in the cultures under the microscope. I haven't noticed any problems with this fertilizer in the tanks. I have been toying with the idea of filtering the phyto out of the culture water, and putting it into NSW but haven't done this yet.
Hope that helps.