the directions iddnt mention running without the membrane in first, but i did run the unit for about an hour before filling up my top off water. It sure seems to waste a lot of water, i would have to say probably 4 gallons for every gallon output. I'll just have to use the waste water to water my plants 
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Fluval Osaka 41g sumpless tank, 250w MH + 130W PC , DIY HOB refugium, Tunze 9002, Koralia 2, Koralia Nano
Toad Stool
Colt Coral
Purple Shrooms
Red Shrooms
Candy Cane Coral
Hammer Coral
Moon Coral
Copper Banded Shrimp..and growing
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