Nope..it shouldn't. I've done this before and didn't have the tank cycle.
If you add some of your tank water into the bucket that you've used to transport the sand, then use a tupperware container to scoop out some of the sand and the water, then lower the container, water and sand into your tank and pour slowly and close to the sand bed...the water might get a bit cloudy but it shouldn't be that bad and its not messy.
Does that make sense?
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