Have you put anything in the tank to create Ammonia? If not drop a cocktail shrimp or fish food in the tank to let it break down. this will tell you if the die off for the 8 hours is going to cause a mini cycle. As for the Zeo part. Zeo will help in the cycle process. but you should still see Ammonia and Nitrite spikes. They maybe short and small.
J
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