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Over time even the smalles residual bacteria can start the nitrogen cycle. It could come from your hands or even airbound bacteria, like if you sneeze or something. If you mix the salt water before storing it sometimes there are nitrates in the salt.
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THE BARQUARIUM:
55 gallon cube - 50 lbs LR - ASM G3 skimmer - 30 Gallon sump - 22 Gallon refugium / frag tank - 4x 24 watt HO T5's - Mag 9.5 return - Pin Point PH monitor - 400 watt XM 20K MH in Lumenarc reflector - Dual stage GFO/NO3 media reactor - 6 stage RODI auto top up -Wavemaster Pro running 3 Koralia 2's.
Fully stocked with fish, corals and usually some fine scotch
http://www.canreef.com/vbulletin/showthread.php?t=55041
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